8XF3

Crystal structure of the reassembled C-phycocyanin octamer from Thermoleptolyngbya sp. O-77

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	I 4 3 2
Unit cell lengths	230.800, 230.800, 230.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.110 - 2.500
R-factor	0.2038
Rwork	0.202
R-free	0.23960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	1.145
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.500	2.500
Number of reflections	36274	5721
<I/σ(I)>	14.3
Completeness [%]	100.0
Redundancy	63
CC(1/2)	0.998	0.554

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.6M sodium chloride, 8% polyethylene glycol 6000, 20% glycerol