8XF0

Crystal structure of the dissociated C-phycocyanin alpha-chain from Thermoleptolyngbya sp. O-77

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 62
Unit cell lengths	169.990, 169.990, 125.968
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.860 - 2.500
R-factor	0.245
Rwork	0.244
R-free	0.28870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.981
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.450	2.450
Number of reflections	149637	23876
<I/σ(I)>	6.3
Completeness [%]	98.6
Redundancy	5.3
CC(1/2)	0.997	0.455

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	15% (w/v) PEG 3350, 10 mM MgCl2 6H2O, 5 mM NiCl2 6H2O, 100 mM HEPES, pH 7.0