8XEY

The Crystal Structure of C-terminal kinase domain of RSK2 from Biortus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.000000
Spacegroup name	P 41 21 2
Unit cell lengths	46.513, 46.513, 294.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.984 - 2.650
Rwork	0.202
R-free	0.28030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.518
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.010	2.780
High resolution limit [Å]	2.650	2.650
Rmerge	0.174	0.994
Number of reflections	10370	1336
<I/σ(I)>	12.4
Completeness [%]	100.0
Redundancy	14.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris pH8.5, 25%PEG3,350