8XET
High-resolution structure of the siderophore periplasmic binding protein FtsB from Streptococcus pyogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-23 |
| Detector | DECTRIS PILATUS3 S 2M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 65 |
| Unit cell lengths | 75.924, 75.924, 102.859 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.500 - 1.110 |
| Rwork | 0.131 |
| R-free | 0.14240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8xeu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.553 |
| Data reduction software | XDS (Feb 5, 2021 BUILT=20210323) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.500 | 1.130 |
| High resolution limit [Å] | 1.110 | 1.110 |
| Rmerge | 0.055 | 0.725 |
| Rpim | 0.013 | 0.290 |
| Number of reflections | 132170 | 6524 |
| <I/σ(I)> | 25 | 2.2 |
| Completeness [%] | 100.0 | 99.3 |
| Redundancy | 18.1 | 8.1 |
| CC(1/2) | 1.000 | 0.751 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 50 mM Zinc sulfate 100 mM MES pH 6.5 25% PEG400 |
