8XE7
Crystal structure of human Sirt2 without Sirt2-specific insertion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-02-24 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 34.893, 69.208, 61.171 |
Unit cell angles | 90.00, 99.34, 90.00 |
Refinement procedure
Resolution | 45.490 - 1.950 |
R-factor | 0.2123 |
Rwork | 0.210 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j8f |
RMSD bond length | 0.008 |
RMSD bond angle | 1.070 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | BALBES |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.490 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.052 | 0.383 |
Number of reflections | 20867 | 1049 |
<I/σ(I)> | 12.5 | 2.7 |
Completeness [%] | 99.2 | 98.4 |
Redundancy | 3.4 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 288 | Bis-Tris (pH6.0), PEG 3350 |