8XE7
Crystal structure of human Sirt2 without Sirt2-specific insertion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.893, 69.208, 61.171 |
| Unit cell angles | 90.00, 99.34, 90.00 |
Refinement procedure
| Resolution | 45.490 - 1.950 |
| R-factor | 0.2123 |
| Rwork | 0.210 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j8f |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BALBES |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.490 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.052 | 0.383 |
| Number of reflections | 20867 | 1049 |
| <I/σ(I)> | 12.5 | 2.7 |
| Completeness [%] | 99.2 | 98.4 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 288 | Bis-Tris (pH6.0), PEG 3350 |
