8XDX
Amylase A from Alkalimonas delamerensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.469, 71.433, 146.535 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.670 - 1.260 |
| R-factor | 0.166 |
| Rwork | 0.166 |
| R-free | 0.18050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.842 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.270 | 1.290 |
| High resolution limit [Å] | 1.260 | 1.260 |
| Rmerge | 0.117 | |
| Number of reflections | 108844 | 1529 |
| <I/σ(I)> | 13.6 | |
| Completeness [%] | 77.4 | |
| Redundancy | 10.4 | |
| CC(1/2) | 0.995 | 0.983 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.2 M sodium fluoride, 0.1 M Bis-Tris propane, 20% (w/v) PEG3350 |
