Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.978530 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.853, 69.782, 83.669 |
| Unit cell angles | 90.00, 89.97, 90.00 |
Refinement procedure
| Resolution | 83.670 - 1.830 |
| R-factor | 0.21627 |
| Rwork | 0.215 |
| R-free | 0.24540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.322 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.670 | 1.870 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.088 | 0.430 |
| Number of reflections | 69672 | 4316 |
| <I/σ(I)> | 8.1 | 2.3 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1M Tris pH 8.5, 10% w/v PEG3350 and 0.2M Magnesium chloride |
