8XC9

Amylase A in complex with acarbose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 31 2 1
Unit cell lengths	140.000, 140.000, 84.180
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.430 - 1.890
R-factor	0.2221
Rwork	0.221
R-free	0.26590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.792
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.430	1.940
High resolution limit [Å]	1.890	1.890
Number of reflections	75774	5369
<I/σ(I)>	14
Completeness [%]	99.6
Redundancy	17.2
CC(1/2)	0.998	0.815

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	0.2 M sodium iodide, 0.1 M BIS-Tris Propane (pH 7.5), 20% (w/v) PEG 3350