8XC8
beta-1,4-galacosyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 84.393, 84.393, 257.495 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.200 - 2.510 |
| R-factor | 0.23863 |
| Rwork | 0.236 |
| R-free | 0.27882 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.340 |
| Data reduction software | XDS (February 5, 2021) |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.200 | 2.870 |
| High resolution limit [Å] | 2.507 | 2.510 |
| Rmerge | 0.142 | |
| Number of reflections | 13430 | 672 |
| <I/σ(I)> | 16.5 | |
| Completeness [%] | 99.8 | |
| Redundancy | 15.8 | |
| CC(1/2) | 0.731 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 1.26M Sodium phosphate monobasic monohydrate, 0.14M Potassium phosphate dibasic |
