8XBN

Crystal structure of Arabidopsis N-amino acetyltransferase NATA1 bound to CoA and HEPES

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.987
Spacegroup name	P 21 21 2
Unit cell lengths	64.320, 76.620, 82.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.280 - 1.350
R-factor	0.15746
Rwork	0.156
R-free	0.18379
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.280	1.390
High resolution limit [Å]	1.350	1.350
Number of reflections	88772	8164
<I/σ(I)>	19.54
Completeness [%]	98.6
Redundancy	13.2
CC(1/2)	0.990	0.410

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.1 M Magnesium chloride hexahydrate, 0.1 M Sodium HEPES pH 7.5, and 10% w/v PEG 4000