8XBC

The substrate binding protein of an ABC transporter in complex with beta-1,3-xylotriose

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	45.610, 84.920, 100.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.300 - 1.360
R-factor	0.2106
Rwork	0.210
R-free	0.23470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.786
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.300	1.400
High resolution limit [Å]	1.360	1.360
Number of reflections	76411	3274
<I/σ(I)>	18.9
Completeness [%]	90.3
Redundancy	10.9
CC(1/2)	0.999	0.733

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	2M ammonium sulfate dibasic, 0.1M sodium acetate/acetic acid, 2% (w/v) PEG 400