8XBA
The substrate binding protein of an ABC transporter in complex with beta-1,4-xylobiose
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-06 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.260, 84.738, 100.119 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.930 - 1.480 |
| R-factor | 0.1709 |
| Rwork | 0.170 |
| R-free | 0.18770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.778 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.680 | 1.560 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.088 | |
| Number of reflections | 63700 | 9298 |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 97.7 | |
| Redundancy | 7.8 | |
| CC(1/2) | 0.998 | 0.755 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.2M ammonium chloride, 20% (w/v) PEG 3350 |
