8XB9
The Crystal Structure of polo-box domain of PLK1 from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.181, 92.786, 35.890 |
| Unit cell angles | 90.00, 101.64, 90.00 |
Refinement procedure
| Resolution | 35.176 - 1.950 |
| Rwork | 0.189 |
| R-free | 0.24480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.423 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.390 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.051 | 0.328 |
| Number of reflections | 15223 | 1066 |
| <I/σ(I)> | 12.7 | |
| Completeness [%] | 98.1 | |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 31% PEG3350, 0.1M Bis Tris pH 5.8 |
