8XAQ

The thermostable and acid-tolerant DNA-binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2019-11-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.99984
Spacegroup name	C 1 2 1
Unit cell lengths	95.074, 38.895, 69.120
Unit cell angles	90.00, 131.66, 90.00

Refinement procedure

Resolution	25.030 - 1.400
R-factor	0.222
Rwork	0.220
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7DI4
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.062	0.410
Number of reflections	36860	36860
<I/σ(I)>	17.54
Completeness [%]	98.4
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	20% (w/v) PEG 1000, 0.1M imidazole, pH 8.0, 0.2M Ca(OAc)2