8XAI
Crystal structure of Protease CPAVM1 in Bacillus subtilis LjM2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-09 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.567, 66.873, 75.605 |
| Unit cell angles | 98.83, 90.16, 100.72 |
Refinement procedure
| Resolution | 37.870 - 1.830 |
| R-factor | 0.2463 |
| Rwork | 0.244 |
| R-free | 0.29310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.256 |
| Data reduction software | autoPROC |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.670 | 1.930 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmeas | 0.700 | |
| Number of reflections | 61551 | 8908 |
| <I/σ(I)> | 1.4 | |
| Completeness [%] | 95.4 | |
| Redundancy | 1.7 | |
| CC(1/2) | 0.970 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294.15 | 0.2 M Ammonium sulfate, 30% w/v Polyethylene glycol 4,000 |
