8XAC

Crystal structure of amidase from Pseudonocardia acaciae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2022-10-28
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.04
Spacegroup name	I 41
Unit cell lengths	165.280, 165.280, 160.612
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.119 - 3.020
Rwork	0.202
R-free	0.25870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.194
Data reduction software	XDS
Data scaling software	pointless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.119	3.100
High resolution limit [Å]	3.000	3.000
Number of reflections	42219	4421
<I/σ(I)>	11.9
Completeness [%]	99.8
Redundancy	14.4
CC(1/2)	0.992	0.727

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG8000, NaCl, CAPS, pH 10.5.