8XAA

Structure of NAP1 in complex with H2A-H2B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9789
Spacegroup name	P 1
Unit cell lengths	79.489, 92.926, 119.354
Unit cell angles	104.10, 106.24, 94.18

Refinement procedure

Resolution	45.930 - 3.350
R-factor	0.2066
Rwork	0.204
R-free	0.26130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.604
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.410
High resolution limit [Å]	3.350	9.080	3.350
Rmerge	0.133	0.029	0.785
Rmeas	0.162	0.036	0.960
Rpim	0.092	0.021	0.543
Total number of observations	126269
Number of reflections	41381	2054	2164
<I/σ(I)>	4.7
Completeness [%]	93.5	92.8	96.7
Redundancy	3.1	3.2	2.9
CC(1/2)	0.988	0.995	0.529

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Tris, pH 8.0, 25 % v/v PEG 400