8X8U

Crystal structure of F.occidentalis GABARAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-27
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 41
Unit cell lengths	53.901, 53.901, 55.665
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.950 - 1.600
R-factor	0.1742
Rwork	0.171
R-free	0.20630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gnu
RMSD bond length	0.011
RMSD bond angle	1.165
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX ((1.20.1_4487: ???))
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.950	1.640
High resolution limit [Å]	1.600	1.600
Rmerge	0.065	1.703
Rmeas	0.068
Rpim	0.019	0.565
Number of reflections	21204	1380
<I/σ(I)>	20.3
Completeness [%]	99.8
Redundancy	12.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Ammonium acetate, 0.1 M Na citrate tribasic dihydrate pH5.6, 24% w/v Polyethylene glycol 4000