8X88
The Crystal Structure of TNIK from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.18071 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 112.750, 112.750, 125.356 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.535 - 2.700 |
| Rwork | 0.198 |
| R-free | 0.25610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.984 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.820 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.055 | 1.106 |
| Number of reflections | 25806 | 3387 |
| <I/σ(I)> | 28.8 | 2.3 |
| Completeness [%] | 100.0 | |
| Redundancy | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.4M Sodium malonate dibasic monohydrate,7 |
