8X7D
Crystal structure of OsHSL1 L204F/F298L/I335F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2023-06-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.019, 140.474, 58.976 |
| Unit cell angles | 90.00, 111.89, 90.00 |
Refinement procedure
| Resolution | 31.550 - 2.200 |
| R-factor | 0.2128 |
| Rwork | 0.210 |
| R-free | 0.24830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.503 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.630 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.068 | 0.404 |
| Number of reflections | 32559 | 1923 |
| <I/σ(I)> | 11.3 | 4 |
| Completeness [%] | 92.7 | 98.2 |
| Redundancy | 5.1 | 5.1 |
| CC(1/2) | 0.998 | 0.863 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | Bis-Tris, PEG 3350, ammonium sulfate |
