8X72
The Crystal Structure of PLK1 from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95365 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 67.500, 67.500, 153.347 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.530 - 2.200 |
| Rwork | 0.195 |
| R-free | 0.24970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.241 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.530 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.111 | 1.003 |
| Number of reflections | 20455 | 1737 |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 96.2 | |
| Redundancy | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.5M MgAc2, 12% PEG 4000, 0.3mM ZnAc2 |
