8X6M
Crystal Structure of Glycerol Dehydrogenase in the Presence of NAD+ and Glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97942 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 131.893, 131.893, 265.259 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.000 |
| R-factor | 0.17736 |
| Rwork | 0.176 |
| R-free | 0.20952 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8gob |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.572 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 118.100 | 2.000 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 78784 | 3865 |
| <I/σ(I)> | 19 | |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 26.1 | |
| CC(1/2) | 0.999 | 0.940 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM Na2HPO4-citrate (pH 4.2), 200 mM NaCl, and 10% (w/v) PEG 3000 |
