8X6K

The X-ray structure of N-terminal catalytic domain of Thermoplasma acidophilum tRNA methyltransferase Trm56 (Ta0931).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-30
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	110.074, 110.074, 57.728
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.030 - 1.802
Rwork	0.165
R-free	0.20240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yy8
RMSD bond length	0.011
RMSD bond angle	1.700
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	5.380	1.910
High resolution limit [Å]	1.800	3.810	1.800
Rmerge	0.051	0.039	0.387
Rmeas	0.053	0.041	0.401
Number of reflections	36948	2602	5811
<I/σ(I)>	30.43
Completeness [%]	98.7
Redundancy	5.3
CC(1/2)	0.999	0.999	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	PEG3350, Bis-Tris