8X6E

crystal structure of the N-terminal TBF1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 2
Unit cell lengths	49.014, 168.635, 39.819
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.375 - 2.000
R-factor	0.213976070355
Rwork	0.213
R-free	0.23630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.578
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.375	2.072
High resolution limit [Å]	2.000	2.000
Number of reflections	23207	1674
<I/σ(I)>	28.2
Completeness [%]	99.9
Redundancy	12.4
CC(1/2)	1.000	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.1 M Tris, pH 8.0, 25 % v/v PEG 400, 10% 0.1 M Magnesium chloride hexahydrate