8X6D
Crystal structure of the C-terminal TBF1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97849 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.794, 72.103, 63.132 |
| Unit cell angles | 90.00, 99.57, 90.00 |
Refinement procedure
| Resolution | 30.310 - 2.000 |
| R-factor | 0.2058 |
| Rwork | 0.204 |
| R-free | 0.23740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.603 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.310 | 2.071 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 33979 | 3354 |
| <I/σ(I)> | 19.2 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.999 | 0.928 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 0.2 M Bis-Tris, pH 5.7, 20% w/v Polyethylene glycol 4,000 |
