8X5Z

The Crystal Structure of PAK1 kinase domain from Biortus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.1806
Spacegroup name	C 2 2 21
Unit cell lengths	51.823, 103.262, 122.182
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.360 - 1.800
Rwork	0.168
R-free	0.21610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.458
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.560	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.055	0.770
Number of reflections	30827	1779
<I/σ(I)>	18.3
Completeness [%]	100.0
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M SPG pH6.0, 25% PEG 1500