8X5O

Crystal structure of the post-fusion core of MjHKUr-CoV spike protein.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	CCD
Collection date	2023-11-09
Detector	RIGAKU
Wavelength(s)	1.5418
Spacegroup name	P 63 2 2
Unit cell lengths	198.736, 198.736, 64.130
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.420 - 2.670
R-factor	0.2433
Rwork	0.241
R-free	0.29150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.489
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.420	2.570
High resolution limit [Å]	2.470	2.470
Number of reflections	26093	2602
<I/σ(I)>	9.5
Completeness [%]	96.8
Redundancy	27.1
CC(1/2)	0.982	0.226

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	2.8M Sodium Acetate, HCl pH 7.0