8X5L

The Crystal Structure of PRKACA from Biortus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97951
Spacegroup name	P 43
Unit cell lengths	77.272, 77.272, 142.698
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.418 - 2.750
Rwork	0.201
R-free	0.25450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.947
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.570	2.900
High resolution limit [Å]	2.750	2.750
Number of reflections	21778	3161
<I/σ(I)>	16.4
Completeness [%]	100.0
Redundancy	11.7
CC(1/2)	0.999	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2M NaAC, 0.1M Na3citrate pH5.5, 10% PEG 4000, 40% v/v Polypropylene glycol P 400