8X4J
Crystal structure of the glycosyltransferase domain of Legionella SetA (unbound)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-07 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 139.413, 139.413, 59.944 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.570 - 2.540 |
| R-factor | 0.19617 |
| Rwork | 0.193 |
| R-free | 0.24681 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.033 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.570 | 2.630 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.010 | |
| Number of reflections | 21215 | 21215 |
| <I/σ(I)> | 48.1 | |
| Completeness [%] | 99.9 | |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 287 | 6% (v/v) MPD, 14% (w/v) PEG 4,000, and 0.1 M sodium/potassium phosphate at pH 6.2 |
