8X3V
Crystal structure of 2C from encephalomyocarditis virus bound with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-15 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 171.000, 171.000, 77.010 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.360 - 2.900 |
| R-factor | 0.229 |
| Rwork | 0.224 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.138 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19_4092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.970 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.186 | 0.952 |
| Rmeas | 0.189 | 0.866 |
| Number of reflections | 15238 | 1108 |
| <I/σ(I)> | 21.2 | 1.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 38.5 | 38.9 |
| CC(1/2) | 0.990 | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 30% PEG400, 0.2 M Sodium citrate tribasic, 0.1 M Tris |
