8X3H

Crystal structure of iron-bound recombinant ovotransferrin N-lobe at 0.93 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-12-10
Detector	RIGAKU RAXIS V
Wavelength(s)	0.7
Spacegroup name	P 21 21 21
Unit cell lengths	46.346, 75.518, 84.647
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 0.930
Rwork	0.103
R-free	0.12370
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	SHELXL

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	0.950
High resolution limit [Å]	0.930	0.930
Rmeas	0.045	0.491
Rpim	0.015	0.188
Number of reflections	190261	7502
<I/σ(I)>	62.5	5.2
Completeness [%]	95.4	76.1
Redundancy	7.8	6.3
CC(1/2)	0.997	0.865

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	100mM HEPES-Na buffer, 21% PEG 3350, 200mM Sodium acetate