8X2S
The Crystal Structure of BPGM from Biortus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-21 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.953743 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.661, 61.524, 122.802 |
| Unit cell angles | 90.00, 95.32, 90.00 |
Refinement procedure
| Resolution | 43.370 - 1.900 |
| Rwork | 0.187 |
| R-free | 0.22930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.299 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.370 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.145 | 0.754 |
| Number of reflections | 44995 | 3090 |
| <I/σ(I)> | 7.2 | 2 |
| Completeness [%] | 99.2 | 97.6 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | HM(P07738) 0.1M Hepes-NaOH pH 7.2, 20% PEG 6000 |
