8X2R
The Crystal Structure of HSP 90-alpha from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-16 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.953698 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 65.712, 88.653, 99.864 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.511 - 1.450 |
| Rwork | 0.126 |
| R-free | 0.15610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.504 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.280 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.061 | 0.871 |
| Number of reflections | 51928 | 3646 |
| <I/σ(I)> | 17.3 | 2.3 |
| Completeness [%] | 100.0 | |
| Redundancy | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | (Index-H6) 0.2M Sodium formate, 20% PEG 3350 |
