8X2A
The Crystal Structure of BMX from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-08 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.953714 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.372, 67.475, 76.519 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.899 - 1.300 |
| Rwork | 0.128 |
| R-free | 0.16060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.723 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.900 | 1.330 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.074 | 0.979 |
| Number of reflections | 70778 | 3312 |
| <I/σ(I)> | 19.2 | |
| Completeness [%] | 99.4 | |
| Redundancy | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Imidazole pH6.5, 20% PEG 8000, 3% MPD |
