8X27
Crystal structure of H5 hemagglutinin from human-infecting H5N8 influenza virus in complex with LSTc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.826, 263.929, 131.199 |
| Unit cell angles | 90.00, 101.08, 90.00 |
Refinement procedure
| Resolution | 31.720 - 3.040 |
| R-factor | 0.2119 |
| Rwork | 0.211 |
| R-free | 0.24180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.130 |
| High resolution limit [Å] | 3.020 | 3.020 |
| Rmerge | 0.149 | 1.424 |
| Number of reflections | 92082 | 8926 |
| <I/σ(I)> | 10.896 | |
| Completeness [%] | 96.6 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 3 % dextran sulfate sodium salt, 0.1 M bicine pH 7.7, 18 % w/v PEG20000 |
