8X24

anti-CRISPR protein-AcrIF25

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	45.343, 67.704, 89.948
Unit cell angles	90.00, 90.44, 90.00

Refinement procedure

Resolution	44.970 - 2.834
Rwork	0.192
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
Data scaling software	Aimless (0.7.7)
Phasing software	PHENIX (1.19.2)
Refinement software	PHENIX (1.19.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.970	2.935
High resolution limit [Å]	2.834	2.834
Number of reflections	12697	1232
<I/σ(I)>	5.48
Completeness [%]	97.0
Redundancy	1.8
CC(1/2)	0.967	0.767

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289.15	PEG335, Sodium sulphate solution