8X23
The Crystal Structure of MAPK13 from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-08 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.95371 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.402, 69.113, 92.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.560 - 1.500 |
| Rwork | 0.124 |
| R-free | 0.17100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.437 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.560 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.072 | 0.833 |
| Number of reflections | 62675 | 3025 |
| <I/σ(I)> | 20 | |
| Completeness [%] | 98.2 | |
| Redundancy | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 50mM (NH4)2 tartrate, 18% PEG 3350 |
