8X1W
CYP725A4 apo structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2021-01-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 196.020, 196.020, 196.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.670 - 2.100 |
| Rwork | 0.238 |
| R-free | 0.26220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond angle | 14.628 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.670 | 2.175 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 35473 | 3677 |
| <I/σ(I)> | 45.9 | 2.7 |
| Completeness [%] | 94.3 | |
| Redundancy | 75.5 | |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 1.6M sodium citrate, pH6.5 |
