8X1K

Structure of RvY_06210 at 2.55 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	62.152, 80.372, 105.025
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.530 - 2.550
R-factor	0.2199
Rwork	0.217
R-free	0.26960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.121
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.170	2.660
High resolution limit [Å]	2.550	2.550
Rmerge	0.096
Rmeas	0.104
Number of reflections	116835
<I/σ(I)>	8.6
Completeness [%]	99.8
Redundancy	6.6
CC(1/2)	0.999	0.618

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.02M Nickel (II) chloride hexahydrate, 0.02M Magnesium (II) chloride hexahydrate, 0.02M cadmium chloride hexahydrate, 0.1M Sodium acetate trihydrate pH 4.5, 24% PEG MME