8X18
Xanthomonas campestris pv. campestris OpgD mutant-D379N with beta-1,2-glucan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 |
| Unit cell lengths | 171.878, 171.878, 128.691 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.723 - 2.250 |
| Rwork | 0.173 |
| R-free | 0.17610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.508 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.723 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 102253 | 5064 |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 10.4 | |
| CC(1/2) | 0.996 | 0.830 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | Bis-Tris HCl (pH5.5), Ammonium sulfate, glycerol, beta-1,2-glucan |
