8X0Z

Crystal structure of A101C/A120C mutant of FfIBP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 41 2 2
Unit cell lengths	69.230, 69.230, 178.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.250 - 2.100
Rwork	0.254
R-free	0.28110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.741
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.250	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.080
Number of reflections	26223	2099
<I/σ(I)>	39.2
Completeness [%]	100.0
Redundancy	25.5
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1 M sodium acetate pH 4.4, 3 M sodium chloride