8X0X
Crystal structure of JE-5C in complex with SARS-CoV-2 RBD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-09-28 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 109.160, 130.340, 131.775 |
| Unit cell angles | 90.00, 105.19, 90.00 |
Refinement procedure
| Resolution | 36.380 - 3.450 |
| R-factor | 0.2426 |
| Rwork | 0.240 |
| R-free | 0.28460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.685 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.160 | 3.570 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Rmerge | 0.127 | 0.426 |
| Rmeas | 0.180 | 0.602 |
| Rpim | 0.127 | 0.426 |
| Number of reflections | 21546 | 2171 |
| <I/σ(I)> | 5.5 | |
| Completeness [%] | 91.3 | 92.45 |
| Redundancy | 3 | 2.9 |
| CC(1/2) | 0.923 | 0.674 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M magnesium sulfate heptahydrate 20 % (w/v) polyethylene glycol 3350 |
