8X0U

Crystal structure of cupin-like fold protein StrC from Stachybotrys sp.g12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	80
Detector technology	CCD
Collection date	2023-05-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979183
Spacegroup name	P 1 21 1
Unit cell lengths	73.700, 253.977, 73.835
Unit cell angles	90.00, 102.79, 90.00

Refinement procedure

Resolution	46.030 - 2.650
R-factor	0.1892
Rwork	0.187
R-free	0.23280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.584
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.030	2.740
High resolution limit [Å]	2.650	2.650
Number of reflections	76171	7554
<I/σ(I)>	8.66
Completeness [%]	99.7
Redundancy	6.9
CC(1/2)	0.988	0.569

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M calcium acetate, 25% PEG3350