8X0T
Frizzled8 CRD in complex with pF8_AC3 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.701, 76.149, 220.245 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.880 - 2.500 |
| R-factor | 0.2397 |
| Rwork | 0.238 |
| R-free | 0.28970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.712 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.880 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.137 | 1.169 |
| Rmeas | 0.142 | |
| Rpim | 0.039 | 0.337 |
| Number of reflections | 21022 | 2074 |
| <I/σ(I)> | 14.99 | 1.97 |
| Completeness [%] | 99.8 | 99.86 |
| Redundancy | 13.1 | 12.8 |
| CC(1/2) | 0.999 | 0.867 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 32% PEG4000, 0.1 M Tris, pH 8.5, 0.2 M MgCl2 |
