8WZS

Crystal structure of SRCRD11 of human DMBT1 from needle-shape crystal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-24
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00
Spacegroup name	P 32 2 1
Unit cell lengths	71.114, 71.114, 55.387
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.180 - 2.540
R-factor	0.17923
Rwork	0.176
R-free	0.24292
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6as5
RMSD bond length	0.008
RMSD bond angle	1.585
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.180	7.510
High resolution limit [Å]	2.540	2.540
Number of reflections	8237	1637
<I/σ(I)>	13.12
Completeness [%]	99.7
Redundancy	5.11
CC(1/2)	0.998	0.634

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293.15	0.2 M Lithium citrate tribasic tetrahydrate, 25% w/v Polyethylene glycol 3,350