8WZL
Arabidopsis thaliana Histone deacetylase 7 (HDA7)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-19 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.308, 74.475, 47.590 |
| Unit cell angles | 90.00, 112.02, 90.00 |
Refinement procedure
| Resolution | 43.900 - 1.500 |
| R-factor | 0.15922 |
| Rwork | 0.157 |
| R-free | 0.19929 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.542 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.900 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 47979 | 2387 |
| <I/σ(I)> | 9.7 | 3.2 |
| Completeness [%] | 98.1 | 96.8 |
| Redundancy | 7.2 | 7.9 |
| CC(1/2) | 0.930 | 0.703 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | 0.1M HEPES, 25% PEG 3350 |
