8WXW
Falcilysin in complex with hemoglobin alpha chain peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-03-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 94.120, 105.720, 125.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.090 - 1.860 |
| R-factor | 0.19 |
| Rwork | 0.189 |
| R-free | 0.20960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.262 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1-5286-0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 1.931 |
| High resolution limit [Å] | 1.600 | 1.864 |
| Rmerge | 0.089 | |
| Rpim | 0.025 | |
| Number of reflections | 104687 | 10280 |
| <I/σ(I)> | 19.1 | |
| Completeness [%] | 99.9 | 99.17 |
| Redundancy | 13.7 | 13.7 |
| CC(1/2) | 0.999 | 0.771 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 4% w/v PEG 1500, 20% v/v glycerol |
