8WXV
Structure of WDR5 in complex with WIN motif containing SET1B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9779 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.904, 61.499, 64.774 |
| Unit cell angles | 110.34, 91.27, 112.39 |
Refinement procedure
| Resolution | 33.540 - 2.400 |
| R-factor | 0.1714 |
| Rwork | 0.168 |
| R-free | 0.20530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.815 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.097 | 0.292 |
| Number of reflections | 23662 | 1168 |
| <I/σ(I)> | 9.47 | |
| Completeness [%] | 98.0 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 0.1 M Bis-Tris, pH 5.5, 25% PEG 3350 |
