8WWF
Crystal structure of (R)-DHPS dehydrogenase HpsO from Ruegeria pomeroyi DSS-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.140, 56.156, 76.185 |
| Unit cell angles | 96.27, 105.96, 90.70 |
Refinement procedure
| Resolution | 41.950 - 1.700 |
| R-factor | 0.15906 |
| Rwork | 0.157 |
| R-free | 0.19412 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.124 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.950 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.139 | 0.356 |
| Number of reflections | 93413 | 4549 |
| <I/σ(I)> | 17.6 | 4.5 |
| Completeness [%] | 95.8 | 93.9 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | 0.8 M Sodium formate, 0.1 M sodium citrate |
