8WWE
Crystal structure of (R)-DHPS dehydrogenase HpsN from Ruegeria pomeroyi DSS-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 68.045, 122.865, 210.235 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.200 - 2.900 |
| R-factor | 0.243 |
| Rwork | 0.242 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.843 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.1_3865) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.200 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.200 | 1.191 |
| Number of reflections | 39957 | 3932 |
| <I/σ(I)> | 20.6 | |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291.15 | 1.1M ammonium sulfate, 0.1M tris |
